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Changelog

scfetch 0.5.0

New features

  • Added ExtractCELLxGENEMeta to extract metadata of CELLxGENE datasets.
  • Added ParseCELLxGENE to download objects from CELLxGENE.
  • Added ExtractHCAMeta to extract metadata of Human Cell Atlas projects.
  • Added ShowHCAProjects to show all available Human Cell Atlas projects.
  • Added ShowCELLxGENEDatasets to show all available CELLxGENE datasets.
  • Added StatDBAttributeto stat database attributes.
  • Added ParseHCA to download objects from Human Cell Atlas.

Minor changes

  • Added filters to ExtractCELLxGENEMeta.
  • Added data.type in ParseGEO to return count matrix for bulk RNA-seq.
  • Added filters to ExtractHCAMeta.
  • Added catalog in ShowHCAProjects.
  • Fixed bug in ParseCELLxGENE and returned failed dataframe when downloading error.

scfetch 0.4.0

New features

  • Added ExportSeurat to convert SeuratObject to other scRNA-seq formats.
  • Added ImportSeurat to convert other scRNA-seq formats to SeuratObject.
  • Added SCEaAnnData to perform data format conversion between SingleCellExperiment and AnnData.
  • Added SCELoom to perform data format conversion between SingleCellExperiment and loom.
  • Added ExtractPanglaoDBComposition to extract cell type composition of PanglaoDB datasets.

Minor changes

  • Added conda.path in ExportSeurat to specify conda enviroment used.
  • Fixed bug in SCELoom to keep gene names and cell ID.
  • Bam2Fastq supported normal bam files (non-10x bam files).
  • Changed function names: ShowPanglaoDBMeta to ExtractPanglaoDBMeta, PrepareZenodo to ExtractZenodoMeta.
  • Supported downloadding normal (non-10x) bam file in DownloadBam.
  • ExtractZenodoMeta supported a vector of Zenodo dois.
  • Added fastq.type in SplitSRA to deal with fastq files from 10x, other scRNA-seq protocols and bulk RNA-seq.
  • Added check for split.cmd.paras in SplitSRA.
  • Added local.data in ExtractPanglaoDBMeta and ExtractPanglaoDBComposition to use cached sample metadata and composition.

scfetch 0.3.0

New features

  • Added ExtractRun to extract run number from GEO.
  • Added DownloadSRA to download SRA according to run number.
  • Added SplitSRA to split SRA to fastqs and format the fastqs to 10x format.
  • Added DownloadBam to download 10x bam files from GEO.
  • Added Bam2Fastq to convert bam to 10x formatted fastqs.

Minor changes

  • Fixed bug in DownloadSRA when downloading bam files.

scfetch 0.2.0

New features

  • Provided APIs for GEO.
  • Added Read10XOnline to load cellranger output.
  • Added ExtractCBComposition to extract cell type composition of UCSC Cell Browser datasets.
  • ParseGEO support creating Seurat object.
  • Added ExtractGEOMeta to support extract metadata from GEO.

Minor changes

  • Supported lazy mode in ShowCBDatasets to save time for multi-runs.
  • Optimized the paras for ExtractCBDatasets and ParseCBDatasets.
  • Added cell.num filter to ParseCBDatasets, ExtractCBDatasets and ShowPanglaoDBMeta.
  • Extracted matrix, barcode and feature info from dataset.json (comprehensive) instead of desc.json.
  • Fixed bugs in ParseCBDatasets when dealing with cellranger output.
  • Added timeout in ParseCBDatasets to avoid possible timeout error.
  • Changed time.out in GEO related functions to timeout.
  • Simplified the output of ParseGEO.
  • Fixed bug in ExtractGEOMeta.

scfetch 0.1.0

  • Provided APIs for Zenodo, PanglaoDB and UCSC Cell Browser.