Generate Reads Profile with deepTools.

ReadProfile(
  bw.folder,
  up.dist = 3000,
  down.dist = 3000,
  species = c("Human", "Mouse", "Rat", "Fly", "Arabidopsis", "Yeast", "Zebrafish",
    "Worm", "Bovine", "Pig", "Chicken", "Rhesus", "Canine", "Xenopus", "Anopheles",
    "Chimp", "E coli strain Sakai", "Myxococcus xanthus DK 1622"),
  gtf.file = NULL,
  compute.matrix.paras = "-p 2",
  plot.heatmap.paras = "--whatToShow 'heatmap and colorbar'",
  deeptools.path = NULL,
  out.folder = NULL,
  out.format = c("pdf", "png", "eps", "svg")
)

Arguments

bw.folder

Folder contains bigWig files.

up.dist

The upstream distance from the TSS. Default: 3000bp.

down.dist

The downstream distance from the TSS. Default: 3000bp.

species

Species used, chosen from "Human","Mouse","Rat","Fly","Arabidopsis","Yeast","Zebrafish","Worm","Bovine","Pig","Chicken","Rhesus", "Canine","Xenopus","Anopheles","Chimp","E coli strain Sakai","Myxococcus xanthus DK 1622". Default: "Human".

gtf.file

GTF file used to create TxDb object. Useful when specie you used is not available in species. Default: NULL.

compute.matrix.paras

Parameters for deepTools computeMatrix. Default: "-p 2".

plot.heatmap.paras

Parameters for deepTools plotHeatmap. Default: "--whatToShow 'heatmap and colorbar'".

deeptools.path

Path contains computeMatrix and plotHeatmap. Default: NULL (conduct automatic detection).

out.folder

Output folder. Default: NULL (current working directory).

out.format

Output heatmap format. Choose from pdf, png, eps and svg. Default: pdf.

Value

NULL.

Examples

# library(DEbPeak) # ReadProfile(bw.folder = path/to/bigWigs, species = "Mouse", deeptools.path = "~/anaconda3/bin")